Phytase blends for enhanced phosphorous mobilization of deoiled seeds

09/03/2022
  Bar chart of the HPLC results. Copyright: © Bio VI Stepwise InsP6 hydrolysis monitored by HPLC.

Congratulations to Anna Joelle Ruff, Kevin Herrmann and Belén Infanzón on their recent publication!

Phytase application in food and feed are challenged by incomplete phytate hydrolysis to inositol. We report a phytase blend of two enzymes with additive effects on phytate (InsP6) hydrolysis and its application in the enzymatic phosphorous recovery process. Blending the fast 6-phytase rPhyXT52 with the 3-phytase from Debaryomyces castellii, which is capable of fully hydrolyzing InsP6, we achieved rapid phosphate release with higher yields compared to the individual enzymes and a rapid disappearance of InsP6-3 intermediates, monitored by HPLC. NMR data suggest a nearly complete phytate hydrolysis to inositol and phosphate. The blend was applied for phosphate mobilization from phytate-rich biomass, such as deoiled seeds. For this emerging application, an up to 43 % increased phosphate mobilization yield was achieved when using 1000 U of the blend per kg biomass compared to using only the E. coli phytase. The phytase blend of Dc phyt/rPhyXT52 was proven very efficient to obtain inositol phosphate depleted meal which has its potential application in animal feeding and is concomitant with the production of green phosphate from renewable resources.

Funding

This work was supported by Ministerium für Wirtschaft, Innovation, Digitalisierung und Energie des Landes Nordrhein-Westfalen (MKW 311-005-1706-0001 / Business-P Project).

Reference

Belén Infanzón, Kevin R Herrmann, Isabell Hofmann, Sabine Willbold, Anna Joëlle Ruff and Ulrich Schwaneberg, Phytase blends for enhanced phosphorous mobilization of deoiled seeds. Enzyme and Microbial Technology (2022), Volume 153. doi.org/10.1016/j.enzmictec.2021.109953