Fe(III)-complex mediated bacterial cell surface immobilization of eGFP and enzymes

17/05/2021
  Lilin Feng, Liang Gao Copyright: © BIO VI Lilin Feng and Liang Gao

Feng, L., Gao, L., Sauer, D. F., Ji, Y., Cui, H., Schwaneberg, U. (2021). Fe(III)-complex mediated bacterial cell surface immobilization of eGFP and enzymes. ChemComm, DOI: 10.1039/d1cc01575c

A facile and reversible method to immobilize a broad range of His6-tagged proteins on the E. coli cell surface through Fe (III)–metal complexes

Bacterial cell surface decoration with proteins is highly attractive for biotechnological process and chemical production. Herein, we report a facile, non-covalent and reversible method to immobilize a broad range of His6-tagged proteins on the surface of E. coli cell through Fe (III)-metal complexes. As a proof of principle, His6-tagged proteins were successfully immobilized on E. coli cell surface: Bacillus subtilis lipase A (BSLA), Candida tropicalis fatty alcohol oxidase (CtFAO), Bacillus licheniformis laccase (BlcotA), Armoracia rusticana horseradish peroxidase (ArHRP) and eGFP. Functionality of the ArHRP was additionally demonstrated by generating a hydrogel around E. coli cells. Being able to immobilize eGFP, four enzymes and generating a hydrogel shell around E. coli prove the versatility of metal complex-based immobilization with broad application potentials in catalysis and biosensing.

Lilin Feng and Liang Gao are supported by PhD scholarships from the China Scholarship Council (CSC No. 201708330279; No. 201708330280). This research was funded by the ‘‘Bundesministerium für Bildung und Forschung’’ (BMBF) (FKZ: 031B0297).

To learn more, please access the full paper on Publications and patents and

Feng, L., Gao, L., Sauer, D. F., Ji, Y., Cui, H., Schwaneberg, U. (2021). Fe(III)-complex mediated bacterial cell surface immobilization of eGFP and enzymes. ChemComm, DOI: 10.1039/d1cc01575c

  Immobilizing proteins through Fe (III)-metal complexes Copyright: © Chemical Communications A facile, non-covalent and reversible method to immobilize a broad range of His6-tagged proteins on the surface of E. coli cell through Fe (III)-metal complexes.