Expanding the industrial use of Robust Oxidative Biocatalysts for the conversion and production of alcohols

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Enzyme Engineering - The Robox Project
The principle of enzyme engineering within the ROBOX project. The Division Molecular Bioeconomy is a partner within this EU H2020 project. For further information please visit

Oxygen functionalities represent the most abundant and important functional groups in chemicals and materials nowadays used and applied in polymers, fine and agro chemicals, vitamins, flavors and fragrances as well as active pharmaceutical ingredients.

The EU funded ROBOX project will demonstrate the techno-economic viability of bio-transformations by developing new robust biocatalysts for the conversion of oxygen functionalities in the pharma, nutrition, fine/specialty chemicals and materials market.

For demonstration four types of robust oxidative enzymes were selected: P450 monooxygenases (P450s), Baeyer-Villiger monooxygenase, alcohol dehydrogenase and alcohol oxidase for which target reactions have already been validated on lab-scale. The collaboration with renowned academic partners and biocatalytic technology providers ensures that the remaining scientific and technological challenges for the development of robust biocatalysts for these reactions will be adequately addressed. By combining cutting edge research and development with a range of targeted demonstrations, this innovation action will bridge the gap between lab and industrial scale in the use of oxidative enzymes for these specific market segments.

The group of Prof. Schwaneberg at Lehrstuhl für Biotechnologie of RWTH Aachen is involved in the evolution of new robust P450 BM3 monooxygenase variants for improved conversion of aromatic precursors for industrial use.


DSM Chemical Technology R&D B.V., The Netherlands

Givaudan Suisse S.A., Switzerland

ChemStream BVBA, Belgium

Dyadic Nederland B.V., The Netherlands

c-LEcta GmbH, Germany

Evocatal GmbH, Germany

LentiKat's a.s., Czech Republic

ACIB GmbH, Austria

Technische Universitaet Graz, Austria

Rijksuniversiteit Groningen, The Netherlands

Universita degli Studi di Pavia, Italy

Rheinisch-Westfaelische Technische Hochschule (RWTH) Aachen, Germany

The University of Manchester, United Kingdom

Danmarks Tekniske Universitet, Denmark

Fraunhofer Gesellschaft zur Foerderung der Angewandten Forschung E.V., Germany

Universitat Autonoma de Barcelona, Spain

Eidgenoessische Technische Hochschule Zurich, Switzerland

Universiteit Maastricht, The Netherlands

PNO Consultants, The Netherlands