Engineering Robust Cellulases for Tailored Lignocellulosic Degradation Cocktails

  Enzyme cocktail digesting celluose Copyright: Int. J. Mol. Sci. Complete cellulose degradation is carried by the joint action of cellulases (endoglucanases, EGLII), cellobiohydrolases (CBH), and β-glucosidases (BG).

The production of biofuels from renewable sources is of high importance nowadays. In the production of biofuels, lignocellulosic biomass can be used as a promising feedstock. The degradation of the lignocellulosic biomass requires harsh conditions of temperature, pressure, and several chemicals. Biocatalysts, in particular cellulases, rise as a green alternative for degradation of biomass. Several types of cellulases are needed to degrade cellulose, the main component of biomass. In order to apply cellulases in the degradation of biomass, more robust and active enzymes are required. In this work, we review protein engineering as a powerful technology to improve cellulases properties, the recent advances in the production of cellulases cocktails, its challenges, and the prospects of tailored cellulases.

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Contreras, F., Pramanik, S., Rozhkova, A. M., Zorov, I. N., Korotkova, O., Sinitsyn, A. P., Schwaneberg, U., Davari, M. D. (2020). Engineering Robust Cellulases for Tailored Lignocellulosic Degradation Cocktails. International Journal of Molecular Sciences, 21(5), 1589.

Project: EnzyBioDeg, funded by BMBF in collaboration with FRC, Russia