Thermostability improvement of endoglucanase by engineering of the disulfide bond


Congratulations to Subrata Pramanik on his recent publication!

  Ribbon diagram of the endoglucanase with engineered disulfide bonds Copyright: © Int. J. Mol. Sci. Ribbon diagram of the endoglucanase with engineered disulfide bonds

In Subrata´s new publication, disulfide bond engineering was successfully applied to improve thermostability of an endoglucanase from Penicillium verruculosum. Endoglucanases are important components of the multienzyme cocktail used for biomass degradation, having huge impact in sustainable bio-economy. The present study showed disulfide bond protein engineering as a potential rational design strategy that could be applicable for improving the thermostability of other enzymes. Subrata is part of the Computational Biotechnology division. This work was realized in the framework of BioEconomoy International and it was possible through funding from German Federal Ministry of Education and Research (BMBF) (FKZ: 031B0506).

Bashirova A., Pramanik S., Volkov P., Rozhkova A., Nemashkalov V., Zorov I., Gusakov A., Sinitsyn A., Schwaneberg U., Davari MD.; Disulfide Bond Engineering of an Endoglucanase from Penicillium verruculosum to Improve Its Thermostability; Int J Mol Sci. 2019. DOI: 10.3390/ijms20071602. shared first authorship.