A peptide‐based coating toolbox to enable click chemistry on polymers, metals, and silicon through sortagging

02/02/2021
 

Congratulations to Maximilian Nöth and Zhi Zou on their recent publication!

In Maximilian’s and Robin’s new publication, a versatile peptide‐toolbox for surface functionalization was established by a combination of a universal anchor peptide (LCI) and sortase‐mediated bioconjugation (sortagging). This toolbox facilitates surface functionalization either as a one‐ or a two‐step strategy. In the case of the one‐step strategy, the desired functionality was directly introduced to LCI. For the two‐step strategy, LCI was modified with a reactive group, which allowed further functionalization (e.g., employing click chemistry). Sortagging of LCI, employing sortase A, was achieved with six different amine compounds: dibenzocyclooctyne amine, biotin‐polyethylene glycol amine, cyanine‐3 amine, kanamycin, methoxypolyethylene glycol amine, and 2,2,3,3,4,4,4‐Heptafluorobutylamine. The purification of LCI‐amine sortagging products was performed by a negative purification using Strep‐tag II affinity chromatography, resulting in LCI‐amine conjugates with purities >90%. For the two‐step strategy, the LCI‐dibenzocyclooctyne sortagging product was purified and enabled, through copper‐free azide‐alkyne click chemistry, universal surface functionalization of material surfaces such as polypropylene, polyethylene terephthalate, stainless steel, gold, and silicon. The click reaction was performed before or after surface binding of LCI‐dibenzocyclooctyne. Finally, in the case of the one‐step strategy, polypropylene was directly functionalized with cyanine‐3 and biotin‐polyethylene glycol amine.

This work was performed in the division Biohybrid Systems and was made possible through funding provided by the BMBF Bioeconomy International project Pro-Planta (FKZ 031B0384) and the Chinese Scholarship Council (CSC No. 201406740053).

To learn more, please access the full paper on Publications and patents and at this link.

M. Nöth, Z. Zou, I. El‐Awaad, L. C. de Lencastre Novaes, G. Dilarri, M. D. Davari, H. Ferreira, F. Jakob, and U. Schwaneberg; A peptide‐based coating toolbox to enable click chemistry on polymers, metals, and silicon through sortagging; Biotechnology and Bioengineering, 2021

‡ These authors contributed equally to the work.

  The two steps: 1. Sortagging and 2. Surface functionalization Copyright: © Wiley VCH  

Figure: Schematic representation of the peptide-based surface functionalization toolbox. The universal anchor peptide LCI is modified with different functional amine compounds through sortagging using sortase A. The latter facilitates surface functionalization of materials such as polypropylene (PP), polyethylene terephthalate (PET), stainless steel, gold, and silicon either through a one‐ or a two‐step strategy. In the case of the one‐step strategy, the desired functionality is directly introduced to LCI, while for the two‐step strategy, LCI is modified with a reactive group, which allows further functionalization (e.g., employing click chemistry). Adapted with permission from Nöth et al. (2021). Copyright Wiley VCH.