Improved sulfation stoichiometry of catechols by the tailored aryl sulfotransferase B

12/03/2019
 

The novel p-NPS-4-AAP assay enables directed evolution campaigns for aryl sulfotransferases, allows direct product detection without any substrate modification, and eliminates less productive variants.

  Scheme of the pNPS-4-AAP assay reaction Copyright: Appl. Microbiol. Biotechnol.

Congratulations to Yu Ji on her recent publication!

In Yu´s new publication, a two-step para-nitrophenylsulfate-4-aminoantipyrine screening system for the laboratory evolution of aryl sulfotransferase B was developed in 96-well microtiter plates to improve the sulfate transfer efficiency toward catechols. A single round of evolution by screening a random mutagenesis library yielded five aryl sulfotransferase B variants with improved 3-chlorocatechol sulfation activity. The variant M5 achieved an up to 4.5-fold improved turnover number and up to 2.3-fold improved sulfate transfer efficiency compared to the wild type enzyme. This research was funded by the China Scholarship Council as well as German Federal Ministry of Education and Research under the projects FuPol and BioSulfa. To learn more, please access the full paper at the journal website of Applied Microbiology and Biotechnology.

Ji, Y., Islam, S., Mertens, A. M., Sauer, D. F., Dhoke, G. D., Jakob, F., Schwaneberg, U. Appl Microbiol Biotechnol, 2019. DOI: 10.1007/s00253-019-09688-0.